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- In Chapter III, found on the corpse between the 2nd and 3rd archways in the Kikimore queen's lair.
- In Chapter IV:
- In Chapter V
Geralt starts the game with one moon rune. Later, they can be purchased from a number of merchants, including the mysterious merchant, Haggard or Thorak in Vergen, or Falas, the Incredible Lockhart or Polycarp of Rinde during Chapter III.
To use a rune, Geralt simply drags it over to one of the rune slots on a suitable sword in the inventory panel.